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. 2007 Feb 26;104(10):3787–3792. doi: 10.1073/pnas.0700115104

Fig. 1.

Fig. 1.

Up-regulation of Bmf RNA levels in anoikis and morphogenesis. (A) Microarray expression profile for Bmf (U133B array, probeset ID 226530_at). Shown is the relative signal intensity for RNA samples from a 3D culture time course, from attached subconfluent (proliferating) cell monolayers (att/pf), from cells cultured in suspension for 24 h (susp), and from confluent (contact-inhibited) cell monolayers (ci). Error bars equal ± SD of three replicate microarray samples. (B) Bmf is up-regulated in suspension and morphogenesis. RT-PCR products for Bmf and GAPDH were derived from MCF-10A RNA samples from attached (att) cells, cells placed in suspension (Left), or cells grown in 3D culture (Right). (C) Bmf is up-regulated in diverse epithelial cell lines upon matrix detachment. RT-PCR products for Bmf and GAPDH were derived from RNA samples from indicated cell lines cultured as attached monolayers (att) or in suspension (sp) for 24 h (HMECtert) or 36 h (all other lines), respectively.