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Detection of male-derived cells in chimeric outgrowths. (a) X-Gal-stained whole mammary fat pad from postpregnant host mouse reveals numerous LacZ+ cells within the gland. (b) After removal of fat, LacZ− cells were found uniformly distributed along the mammary ducts in this whole mount. (c) LacZ+ cells in a whole mount from a second-generation transplant showing the self-renewal of male progeny. (Scale bars: 1.0 mm.)
