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. 2007 Feb 21;104(10):3990–3995. doi: 10.1073/pnas.0611624104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 3. — Quantification of UC-1V150 molecules conjugated to MSA. (A) Different concentrations of UC-1V150-SANH were subjected to spectrophotometric analysis. (B) The readout of UC-1V150-SANH was used to derive a standard curve. (C) Conjugation of UC-1V150 to MSA was indicated by a UV absorption peak at 342 nm due to hydrazone formation. The concentration of UC-1V150/MSA used in this analysis at 0.25 μg was based on MSA concentration, which is equivalent to 3.63 μM MSA. The concentration of UC-1V150 in conjugate was calculated as (0.6901 − 0.0056)/0.0366 = 18.7 μM; thus, the UC-1V150:MSA ratio is 18.7/3.63 = 5.2.