Figure 2.

cDNA inserts representing candidate clusters C1 (A), C2 (B), and C5 (C) and from the PSSPP (D) were hybridized to RNA dot blots (Human RNA Master blot, CLONTECH) containing mRNAs from 50 normal human cell types or tissues. The mRNA dots are from whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantial nigra, temporal lobe, thalamus, subthalamic nucleus, spinal cord, heart, aorta, skeletal muscle, colon, bladder, uterus, prostate (position C7 in A–D), stomach (position C8), testis, ovary, pancreas, pituitary gland, adrenal gland, thyroid gland, salivary gland (D7), mammary gland, kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lymph node, bone marrow, appendix, lung (F2), trachea (F3), placenta, fetal brain, fetal heart, fetal kidney, fetal liver, fetal spleen, fetal thymus, and fetal lung. Each EST clone (Genome Systems, St. Louis) was first confirmed by sequencing, and the clone inserts were isolated as EcoRI–NotI fragments and labeled with ³²P (Lofstrand Laboratories, Gaithersburg, MD).