Fig. 2.

Generation of human SP-C (hSP-C) driven WT cyclin E or degradation-resistant (T62A/T380A) hcyclin E transgenic lines. (A) The cyclin E transgenic constructs. Restriction endonuclease sites used for cloning these species are shown. (B) Exogenous hcyclin E immunoblot expression profiles in normal lung tissues of transgenic mice. Actin expression served as a loading control. (C) Immunohistochemical detection of hcyclin E in lung tissues of a representative WT (line 2) transgenic cyclin E mouse and a nontransgenic (Tg⁻) control mouse that did not stain. The left and right arrows in Right indicate hcyclin E nuclear staining in representative pneumocyte and bronchiole epithelial cells, respectively.