Fig. 5.

Enhanced recruitment of cytokines and neutrophils into mouse peritoneum injected with the lpxF mutant XWK4. In these experiments, 10⁶ viable bacteria were injected into each mouse peritoneum. After 4 h, the peritoneal fluid was withdrawn. (A) Cytokines tested were TNFα, macrophage inflammatory protein 2 (MIP2), MCP1, IL6, IL13, IL1β, and keratinocyte-derived chemokine. The cytokine levels were determined by the Elisa Tech Company (Aurora, CO). Six mice were used in each experiment; three were infected with U112 and three with XWK4. The experiment was repeated three times and the data averaged. (B) The percentage of neutrophils in the peritoneal fluids was determined. Six mice were used in each experiment; three were injected with U112 and three with XWK4. The experiment was repeated twice and the data averaged. (C) The titers of viable bacteria in the fluids were determined by serial dilution plating. The P value was <0.02 for all observed differences.