Fig. 2.

Structure of chimeric gDs present in R5322 or designed for construction of recombinant R5321 or R5323 viruses and the properties of the recombinant viruses. (A) The sequence arrangement of gD present in R5322 or constructed for other recombinant viruses. (B) Replication of recombinant viruses in J-nectin, J-HveA, or Vero-13R cell lines. Cells grown in 25-cm² flasks were exposed to 0.1 pfu of the recombinant virus per cell and harvested 24 h after infection. Progeny virus was titrated on Vero-13R cells. (C) Photograph of electrophoretically separated proteins from lysates of cells infected with R5321, R5322, and R5323 recombinant viruses. Vero-13R cells grown in 25-cm² flasks were exposed to 1.0 pfu of R5321, R5322, and R5323 virus per cell. The cells were harvested 24 h after infection, solubilized, subjected to electrophoresis in 10% denaturing polyacrylamide gels, electrically transferred onto a nitrocellulose sheet, and reacted with a monoclonal antibody against ICP0, gD (ZC25), uPA, and IL-13, respectively.