Fig. 4.
Coprecipitation of the polypeptides consisting of the N-terminal uPA–gD33–60 and the C-terminal gD219–314 domains. (A) Structure of the plasmids transfected in a pairwise manner into HEK293 cells. The transfected cells were harvested 40 h after transfection. (B and C) Portions comprising 10% of the total cell lysates were solubilized, subjected to electrophoresis in a denaturing gel, and probed with antibody to uPA (Ba and C Left), IL-13 (Ba), or gD (Ba) or with antibody to Myc (Bb and C Right). Other aliquots of the lysates were reacted with antibodies to gD, uPA, or IL-13. The precipitates were collected, solubilized, subjected to electrophoresis in denaturing gels, and reacted with antibody to uPA (Bc) or Myc (Bd).