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. 2007 Feb 27;104(10):4159–4164. doi: 10.1073/pnas.0611236104

Fig. 3.

Fig. 3.

CK1 inhibitor IC261 abolishes CK1ε-271-dependent Aβ40/Aβ42 increase in a dose- and time-dependent manner. N2A cells stably expressing APP-695 were transiently transfected with CK1ε-271. (A) IC261 dose response (3 h). Forty-eight hours after transfection, cells were incubated in the absence or presence of various concentrations of CK1 inhibitor IC261. Cell supernatants were collected after 3 h of incubation and subjected to Aβ40 (Left)/Aβ42 (Right) ELISAs. (B) IC261 time response (50 μM). Forty-eight hours after transfection, CK1 inhibitor IC261 (50 μM) was added. Cell supernatants were collected at different times of incubation and subjected to Aβ40 (Left)/Aβ42 (Right) sandwich ELISAs. Data for at least three experiments (mean ± SEM.) were compared (GraphPad Prism ver. 4.0) with the control conditions (no drug or zero time point). ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; one-tailed Student's t test, 95% significance level.