Fig. 2.

Ischemia enhances REST binding to the MOR-1 promoter in CA1. (a) ChIP analysis of cross-linked chromatin from the CA1 and CA3 of control (Ctl) and experimental animals at 12, 24, and 48 h after ischemia immunoprecipitated with an anti-REST antibody. Immunoprecipitation with nonimmune IgG showed negligible PCR product, indicating specificity of the ChIP technique. Left lanes show ODN length standards. (b) Band densities for PCR products were normalized to that of the corresponding control input. Bars represent mean ± SEMs. ∗, P < 0.05 (Student's unpaired t test).