Fig. 2.

Knockdown of PSD-95 prevents the developmental increase in synapses with functional AMPA-Rs. (a) AMPA-mEPSCs recorded at −60 mV from CA1 neurons at 6 Div and 9 Div (one control and one expressing Hp1 for 3 days). (Scale bars: 20 pA and 50 ms.) (b) The increase in mEPSC frequency from 0.36 ± 0.06 Hz (n = 13) to 0.83 ± 0.12 Hz (n = 11) (P < 0.001) was prevented by Hp1 (0.46 ± 0.06 Hz, n = 11, P = 0.01 vs. 9 Div). (c) There was no difference in average mEPSC amplitude in a cell-wise comparison (6 Div: 10.7 ± 0.2 pA, n = 13; 9 Div Con: 11.0 ± 0.5 pA, n = 11; 9 Div Hp1: 9.9 ± 0.6 pA, n = 11; P > 0.15).