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. Author manuscript; available in PMC: 2007 Mar 12.
Published in final edited form as: Mol Genet Metab. 2006 Sep 7;90(1):97–111. doi: 10.1016/j.ymgme.2006.07.011

Fig. 3.

Fig. 3

TGIF HPE mutants interact with corepressors. (A and B) COS1 cells were transfected with His6-CtBP and Flag-TGIF wildtype or HPE mutant, as indicated. Protein complexes were isolated on anti-Flag agarose and analyzed by western blot for the presence of co-precipitating CtBP. A portion of the lysates was subjected to direct western blot analysis to monitor protein expression (below). (C) COS1 cells were transfected with Myc-epitope tagged Sin3 expression construct, together with the indicated Flag-tagged TGIF constructs or control vector. Protein complexes were isolated on anti-Flag agarose and analyzed by western blot for the presence of co-precipitating Sin3. A portion of the lysates was subjected to direct western blot analysis to monitor protein expression (below).