Figure 1.

Notch and BMP signaling components in the embryonic cerebellar primordium. Sagittal cryosections of E12.5 wild-type brains were stained by in situ hybridization with antisense probes for Notch1, Mash1, Hes5, Msx2, Math1 and Delta1. Differentiating neural precursors in the ventricular zone (VZ) and rhombic lip (RL) are delineated by Mash1 and Math1, respectively. Msx2 expression is indicative of BMP receptor signaling, and overlaps with the Math1⁺ territory at this stage. Hes5 and Delta1 are expressed throughout the cerebellar progenitor population, and are generally indicative of high and low Notch1 activity, respectively. The field shown in these panels corresponds to the box in the schematic (inset). Scale bar represents 300 μm.