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Kinetic parameters of recombinant TcMCPs were determined from Lineweaver–Burk plots, as described in the Materials and methods section. N.D., not detected.
| Substrate | Co2+ (50 μM) | Km (mM) | kcat (s−1) | kcat/Km (s−1·mM−1) |
|---|---|---|---|---|
| TcMCP-1 | ||||
| FA-Ala-Lys | No | 0.166 | 20 | 121 |
| FA-Ala-Lys | Yes | 0.169 | 5.7 | 34 |
| FA-Phe-Phe | No | N.D.* | N.D.* | N.D.* |
| FA-Phe-Phe | Yes | 0.073 | 0.7 | 9.6 |
| TcMCP-2 | ||||
| FA-Phe-Phe | No | 0.024 | 7.6 | 315 |
| FA-Phe-Phe | Yes | 0.034 | 11 | 323 |
