Figure 5.

The PDZ binding motif of PTEN affects protein stability. (A) Schematic diagram of PTEN mutants. WT, wild type. (B) Coimmunoprecipitation experiments were performed in 293T cells transfected with the indicated plasmids as described in Fig. 1. IP, immunoprecipitation. (C) Rat-1 (Upper) and LNCaP (Lower) cells were infected with retrovirus expressing the indicated PTEN mutant, then selected in G418. Lysates were analyzed for expression of FLAG-PTEN at multiple time points. Results from the 25-day time point are shown.