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Various concentrations of full-length ovine recombinant ARQ (filled symbols) or VRQ (open symbols) PrP protein was captured by anti-PrP mAb FH11-coated (circles) or A516-coated (squares) ELISA plates as described in the the Materials and methods section. Recombinant PrP was detected by 0.25 μg/ml purified anti-PrP mAb 245. Results shown are TRF cps±S.D. for triplicate wells.
