Figure 7. Detection of ovine PrP by Western blot.

Homozygous VRQ scrapie-free (upper panel) and scrapie-infected (lower panel) brain material was prepared as described in the Materials and methods section. Brain homogenate was treated with various concentrations of PK and analysed by SDS/PAGE and Western blot using anti-PrP mAb 683; 600 μg wet tissue equivalent was loaded per track. Molecular mass markers (kDa) are shown on the left-hand side.