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. 2006 Dec 21;401(Pt 2):559–567. doi: 10.1042/BJ20061081

Figure 6. The p38 pathway increases the transcription of ATF3 gene.

Figure 6

(A) Immortalized MEFs were treated with anisomycin for 40 min. Nuclear RNAs (for pre-mRNA detection, lanes 1–5) and total RNAs (for mature mRNA detection, lanes 6–9) were isolated and analysed by RT–PCR using the indicated primer sets. The intron–exon primers hybridize to intron 1 and exon B; they detect the ATF3 pre-mRNA and genomic DNA (G. DNA) but not mature mRNA. The control primers hybridize to exon C and exon E; they detect the mature ATF3 mRNA. (B) Immortalized MEFs were pretreated with SB203580 (SB) for 30 min, followed by anisomycin treatment for 40 min and analysed by RT–PCR using the intron–exon primers to detect ATF3 pre-mRNA (as indication of transcription). (C) Immortalized MEFs were transfected with DNAs expressing β-Gal or MKK6(CA) for 36 h and analysed by RT–PCR as in (B).