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. 2006 Dec 21;401(Pt 2):559–567. doi: 10.1042/BJ20061081

Figure 7. The p38 pathway induces ATF3 gene expression at least in part via the transcription factor CREB.

Figure 7

(A) The conserved regions of the human (h) and mouse (m) ATF3 promoter contain potential binding sites for several transcription factors known to be phosphorylated by p38: CREB, C/EBPβ, gadd153/Chop10, MEF2A and MEF2C. (B) Immortalized MEFs were transfected with DNA expressing β-Gal, CREB, C/EBPβ, gadd153/Chop10, MEF2A, MEF2C or MKK6(CA) as indicated for 36 h and analysed by immunoblot. (C) Left panel: immortalized MEFs were transfected with DNA expressing β-Gal, CREB, MKK6(CA), or MKK6(CA) plus A-CREB (a DN CREB) for 36 h and analysed by immunoblot. Right panel: immortalized MEFs were transfected with DNA expressing β-Gal or increasing amounts of A-CREB for 36 h, followed by anisomycin treatment for 3 h and analysed by immunoblot. (D) Immortalized MEFs were transfected with DNA expressing β-Gal, MEF2C, MKK6(CA), or MKK6(CA) plus DN MEF2C (R24L or R3T) for 36 h and analysed by immunoblot. Shown in (B–D) are representative results of three repeats.