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. 2006 Dec 21;401(Pt 2):497–503. doi: 10.1042/BJ20060536

Figure 1. Identification of free nitrotyrosine O-[35S]sulfate generated and released by HepG2 human hepatoma cells metabolically labelled with [35S]sulfate in the presence of nitrotyrosine.

Figure 1

Autoradiograph taken from the TLC plate used for the TLE analysis. Confluent HepG2 cells were incubated in the labelling media containing 0, 5, 10, 25 or 50 μM nitrotyrosine for 18 h as indicated. The broken-line circles indicate the position of nitrotyrosine O-[35S]sulfate (NitroTyrS).