Figure 3. Lineweaver–Burk double-reciprocal plots of SULT1A3 with L-p-tyrosine as substrate in the absence or presence of different concentrations of nitrotyrosine.

Concentrations of nitrotyrosine tested were 0 (□), 125 (■), 250 (▲) and 500 (●) μM. Concentrations of L-p-tyrosine were in mM and velocities were expressed as nmol of sulfated product formed/min per mg of protein. Results are means for three determinations. The inset shows an enlargement of the region where the lines converge in the plot.