Figure 4. Generation and release of free nitrotyrosine O-[35S]sulfate by HepG2 human hepatoma cells labelled with [35S]sulfate in the presence of SIN-1.
Autoradiograph taken from the TLC plate used for the TLE analysis. Confluent HepG2 cells were incubated for 18 h in the labelling media containing 0, 0.5, 1 and 2.5 mM SIN-1 (lanes 2–5 respectively). Lane 1 shows the nitrotyrosine O-[35S]sulfate generated in the enzymatic assay. The broken-line circles indicate the position of nitrotyrosine O-[35S]sulfate (NitroTyrS).