Table 2. Kinetic constants of the sulfation of nitrotyrosine and dopamine by human SULT1A3.
Substrate | Vmax (nmol/min per mg of protein) | Km (μM) | Vmax/Km |
---|---|---|---|
L-Nitrotyrosine (without L-tyrosine) | 4.2±0.5 | 130.5±15.3 | 0.032 |
L-Nitrotyrosine (with 250 μM L-tyrosine) | 3.7±0.2 | 117.3±6.1 | 0.032 |
L-Nitrotyrosine (with 500 μM L-tyrosine) | 3.8±0.6 | 129.4±26.9 | 0.029 |
Dopamine | 52.7±4.1 | 6.5±0.7 | 8.11 |