Table 3. Kinetic constants of the sulfation of nitrotyrosine and dopamine by human SULT1A3 at different pH values.
Vmax (nmol/min per mg) | Km (μM) | Vmax/Km | |
---|---|---|---|
Sulfation of nitrotyrosine | |||
pH 5.5 | 0.9±0.2 | 144.4±30.3 | 0.006 |
pH 7.0 | 4.2±0.5 | 130.5±15.3 | 0.032 |
pH 9.0 | 0.3±0.1 | 164.1±31.6 | 0.002 |
Sulfation of dopamine | |||
pH 7.0 | 52.7±4.1 | 6.5±0.7 | 8.11 |
pH 9.0 | 130.6±8.7 | 38.1±2.6 | 3.43 |
pH 11.0 | 101.3±18.5 | 31.6±12.0 | 3.21 |