Table 4. Amounts of free [3H]nitrotyrosine and [3H]nitrotyrosine O-sulfate generated by HepG2 human hepatoma cells labelled with [3H]tyrosine in the presence of SIN-1.
Results shown represent the counts (c.p.m.×10−3) corresponding to free [3H]nitrotyrosine or [3H]nitrotyrosine O-sulfate present in the 250 μl cell lysate or labelling medium samples. Results shown are calculated means±S.D. for three experiments. Percentage values in the parentheses indicate the ratio between free [3H]nitrotyrosine and [3H]nitrotyrosine O-sulfate present in cell lysate or medium samples at individual time points.
| [3H]Nitrotyrosine | [3H]Nitrotyrosine O-sulfate | |
|---|---|---|
| Cell lysate | ||
| 1 h | 29.2±4.4 (94.8%) | 1.6±0.5 (5.2%) |
| 3 h | 28.3±1.2 (93.4%) | 2.0±0.1 (6.6%) |
| 18 h | 25.4±3.2 (90.4%) | 2.7±0.8 (9.6%) |
| Medium | ||
| 1 h | 88.9±6.8 (76.8%) | 26.9±4.2 (23.2%) |
| 3 h | 91.8±25.8 (74.9%) | 30.8±1.4 (25.1%) |
| 18 h | 33.5±15.9 (40.8%) | 48.7±4.0 (59.2%) |