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. 2007 Feb 13;5(3):e43. doi: 10.1371/journal.pbio.0050043

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© 2007 Nadel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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SJ (primer combination 3 + 2). Top and bottom lanes depict sequences before recombination (heptamers, spacers and nonamers are specified). Recombined clones are depicted between top and bottom lanes, with homology to the unrecombined sequences indicated by vertical lines. Lower cases represent reactive RSS involved in the trans-V(D)J recombination reactions (specified as donor RSS and target RSS). Upper cases represent coding segments, or bystander RSSs in the ESJ behaving like coding segments (specified as bystander RSSs). Italics indicate potential P nucleotides; bold type, N nucleotides; heptamers and nonamers are underlined; identical sequences shown on separate lanes are issued from distinct transfections and represent therefore independent junctions. Since identical sequences were often obtained in a given transfection, this representation might in some cases bias the representation towards processed junctions (because they acquire distinguishing features).