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. 2007 Feb 13;5(3):e43. doi: 10.1371/journal.pbio.0050043

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© 2007 Nadel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of the comparative trans-V(D)J recombination assay. Left panel: recombination of an ESJ 23-RSS (Jδ1/Dδ3 or Dβ1Δ) with a 12-RSS target (Jβ2.7); Right panel: recombination of a coding segment/23-RSS (Dδ3 or Dβ1) with a 12-RSS target (Jβ2.7). The expected breakpoints are shown for each case.

(B) Typical example of semiquantitative PCR/PE assays obtained from the (Jδ1/Dδ3) ESJ × Jβ2.7 versus Dδ3 × Jβ2.7 comparisons. PE assays shown were performed on serial dilutions of PCR products.