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. Author manuscript; available in PMC: 2007 Mar 13.
Published in final edited form as: Gastroenterology. 2006 Sep;131(3):878–884. doi: 10.1053/j.gastro.2006.06.013

Figure 1.

Figure 1

Ad-siRNA effectively inhibited radixin expression in collagen sandwich cultured rat hepatocytes. (A) Clone 9 cells were treated with PBS, Ad-siControl, or the four siRNAs individually or in various combinations at a virus dose of MOI 10. The cells were harvested 3 days after treatment and cell lysates were separated by PAGE, and blotted for radixin and normalized to β-actin. (B) Rat hepatocytes treated with PBS, Ad-siControl or Ad-siRDXC were harvested 1d, 3d and 5d after the treatment and cell lysates were blotted for radixin and β-actin. (C) Hepatocytes were treated with PBS, Ad-siControl or Ad-siRDXC with MOI 25 or 50, harvested on day 5, and cell lysates were blotted for radixin and β-actin. (D) The hepatocyte lysates were also blotted for Mrp2, Bsep, Mrp3 and Mdr1. A representative result of at least three experiments is shown.