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. Author manuscript; available in PMC: 2007 Mar 13.
Published in final edited form as: Gastroenterology. 2006 Sep;131(3):878–884. doi: 10.1053/j.gastro.2006.06.013

Figure 4.

Figure 4

Ad-siRadixin altered the localization of apical bile transporters in hepatocytes. Hepatocytes were treated with PBS, Ad-siControl or Ad-siRadixin and cultured for 5 days. Cells were double labeled with anti-Mrp2 and anti-Bsep antibodies (top 2 rows), with anti-Mdr1 (third row), or anti-Oatp2 antibodies (bottom row). Note that all three apical membrane transporters, but not the basolateral membrane protein, showed significant intracellular localization in knock down areas after Ad-siRadixin treatment. Arrow points to collapsed canaliculi. Bar =10 μm