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. Author manuscript; available in PMC: 2007 Mar 13.
Published in final edited form as: J Biol Chem. 2006 Jan 1;281(10):6691–6698. doi: 10.1074/jbc.M508314200

FIGURE 3. The phagosome is a site of LTC4 biosynthesis.

FIGURE 3

A, kinetics of LTC4 generation by mouse peritoneal macrophages stimulated with zymosan (10 particles/cell). B, peritoneal macrophages isolated from BALB/c mice were stimulated with unopsonized zymosan (10 particles/cell) for 15 and 60 min, fixed in 2% paraformaldehyde, permeabilized with 0.025% saponin, and incubated with specific antibodies for each of the enzymes of LTC4 biosynthesis followed by FITC-conjugated secondary antibodies. C, to demonstrate LTC4 formation at the phagosome, the cells were fixed with EDAC and immunostained with rat mAb anti-Cys-LTs followed by Cy3-conjugated donkey anti-rat IgG. Immunofluorescence images are paired with the corresponding Nomarski or bright field image.