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. Author manuscript; available in PMC: 2008 Feb 3.

Published in final edited form as: Biochem Biophys Res Commun. 2006 Dec 11;353(1):189–194. doi: 10.1016/j.bbrc.2006.12.018

Fig. 3 — GAS6 promoter contain an ERE (estrogen response element) interacting with ERα. a). An ERE site was identified in the GAS6 promoter by DRAGON ERE FINDER. The underlined ERE site along with adjacent sequence is shown. b).Binding of ERα to the ERE of GAS6 gene in vitro. The GAS6 ERE oligonucleotide as a probe was incubated with 10 μg of nuclear extracts prepared from MCF-7 cells in the presence of unlabeled specific or non-specific competitors. The nuclear extracts were incubated with anti-ERα antibody as indicated or control IgG before being added to the probe. Specific DNA-protein complex is indicated by an arrow. c). ERα binds to GAS6 promoter in vivo. Chromatin extracts were made from MCF-7 cells following treated with or without 10nM E2 for 45 min and ChIP assays were performed using an antibody against ERα, or control IgG. DNA fragments were extracted from immunoprecipitates. The GAS6 and pS2 promoter regions containing EREs were amplified.

Fig. 3 — GAS6 promoter contain an ERE (estrogen response element) interacting with ERα. a). An ERE site was identified in the GAS6 promoter by DRAGON ERE FINDER. The underlined ERE site along with adjacent sequence is shown. b).Binding of ERα to the ERE of GAS6 gene in vitro. The GAS6 ERE oligonucleotide as a probe was incubated with 10 μg of nuclear extracts prepared from MCF-7 cells in the presence of unlabeled specific or non-specific competitors. The nuclear extracts were incubated with anti-ERα antibody as indicated or control IgG before being added to the probe. Specific DNA-protein complex is indicated by an arrow. c). ERα binds to GAS6 promoter in vivo. Chromatin extracts were made from MCF-7 cells following treated with or without 10nM E2 for 45 min and ChIP assays were performed using an antibody against ERα, or control IgG. DNA fragments were extracted from immunoprecipitates. The GAS6 and pS2 promoter regions containing EREs were amplified.