Figure 2.

The GMM phenotype is primarily due to increased cell growth. Control GMR-Gal4/+ (A,D,G,J), GMM (B,E,H,K), and GMM, lid¹⁰⁴²⁴/+ (C,F,I,L) eye imaginal discs from wandering third instar larvae. (A–C) Anti-Elav to detect differentiating neurons posterior to the morphogenetic furrow (MF; marked with white bar). (D–F) BrdU incorporation to visualize cells in S phase. (G–I) Cell death visualized by expression of cleaved Caspase-3. (J–L) Cortical actin visualized by phalloidin-Rhodamine conjugate. Eye discs are orientated with anterior to the left.