Figure 1.

Ecsit isoform 1 copurifies with tandem affinity-purified NDUFAF1. (A) Ten Ecsit splice isoforms are predicted by the EBI alternative splicing database, of which two are identified in experimental studies (isoforms 1 and 2). Shown are the exons (vertical bars) and introns (horizontal lines) that comprise the complete transcript. The Swissprot annotation and predicted molecular mass are indicated when available. (B) FT-MS sequence coverage of Ecsit in eluates of tandem affinity-purified NDUFAF1 in HEK293 mitochondria demonstrates the presence of Ecsit isoform 1. Identified peptides are indicated in bold and in boxes, whereas exon 6, unique for Ecsit isoform 1, is indicated in gray.