Figure 2.

Western blot of total protein isolated from the rosettes of wild-type Arabidopsis plants at various stages of development. A, Protein blot probed with TAG lipase antiserum. Lane 1, Total protein isolated from 2-week-old rosette leaves; lane 2, total protein isolated from 3-week-old rosette leaves; lane 3, total protein isolated from 4-week-old rosette leaves; lane 4, total protein isolated from 5-week-old rosette leaves; lane 5, total protein isolated from 6-week-old rosette leaves. Each lane was loaded with 10 μg of protein. B, Chlorophyll levels in developing and senescing rosette leaves. Age of the leaves (weeks after planting) is indicated.