Western blot of total protein extracts from organs of 6-week-old wild-type Arabidopsis plants. The blot was probed with TAG lipase antiserum. Lane 1, Total protein isolated from roots; lane 2, total protein isolated from inflorescence stems; lane 3, total protein isolated from cauline leaves; lane 4, total protein isolated from developing siliques; lane 5, total protein isolated from flowers. Each lane was loaded with 10 μg of protein.