Table I.

TAG lipase activity of recombinant fusion proteins MBP-LipF and MBP-LipS

Lipase activity was measured by iodine-stained TLC and a NEFA-C assay. Values are means ± se for n = 3. Means identified by different superscripts are significantly different at the 5% level based on Duncan's multiple range test. Percentages relative to the pMal-c2 control are indicated. MBP, Maltose binding protein control produced from the empty pMal-c2 vector; MBP-lipF, full-length lipase fused to MBP; MBP-lipS, truncated lipase (lacking predicted transit peptide) fused to MBP.

Recombinant Protein	TLC Assay		NEFA-C Assay
	Density Relative to Background × 104	% Increase	mmol FFA/μg Protein	% Increase
MBP	8.50a ± 0.30	–	23.3a ± 2.04	–
MBP-lipF	9.17a ± 0.30	7.9	26.0a,b ± 2.06	11.3
MBP-lipS	11.0b ± 0.05	29.5	33.3b ± 1.79	42.5