Figure 4.

VvMYBPA1 activates promoters of flavonoid pathway genes involved in PA synthesis. The MYB transcription factors and promoters used for transfection of grape cell cultures are indicated. Control indicates the activity of the respective promoter transfected without a MYB factor. Each transfection (except E, MybPA1 without bHLH) contained the 35S∷EGL3 construct encoding the bHLH protein EGL3 (GenBank accession no. NM20235) from Arabidopsis and as internal control the Renilla luciferase plasmid pRluc (Horstmann et al., 2004). The normalized luciferase activity was calculated as the ratio between the firefly and the Renilla luciferase activity. Each column represents the mean value of three independent experiments with error bars indicating ±ses.