Figure 5.

Map-based cloning of IGN1 and complementation test. A, Genetic linkage map of the IGN1 region on a part of the short arm of L. japonicus chromosome 5. The rough map position of IGN1 is also given in the previous article (Sandal et al., 2006). B, Candidate genes in the region between dCAPS markers, 1235c1 and 1235b2. I, β-Ketoacyl-CoA synthase; II, protein phosphatase 2C; III, IGN1; IV, hypothetical protein; V, pentatricopeptide repeat-containing protein. C, Complementation of the ign1 mutant with an NheI-XbaI fragment containing the complete wild-type IGN1 gene. From left to right: wild type (Gifu), ign1, ign1 transformed with empty vector, ign1 transformed with IGN1 gene. Plants were grown under nitrogen-free conditions and harvested 19 d after inoculation with M. loti. Bar represents 10 mm.