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Complementation of ign1 mutant with an NheI-XbaI fragment containing the complete IGN1 gene
Plants were grown with nitrogen-free medium and harvested 19 d after inoculation with M. loti. Data are the means of 12 plants each.
| Line | Shoot Length | C2H2 Reduction Activity |
|---|---|---|
| mm ± sd | nmol/h/plant ± sd | |
| Gifu | 32.46 ± 4.03 | 70.51 ± 18.4 |
| ign1 | 16.92 ± 3.95 | 02.51 ± 4.54 |
| ign1/empty vector | 14.33 ± 1.61 | 01.62 ± 2.17 |
| ign1/NheI-XbaI fragment | 34.31 ± 4.35 | 73.51 ± 18.38 |
