Figure 3.

HR-mediated resistance in the different Arabidopsis lines. Four-week-old plants, wild type, and same GSNOR transgenic lines as those in Figure 1 were treated by avirulent pathogens, Pp isolate Cala2 (A) or Psm avrRpm1 (B). A, 10 μL droplet of Pp Cala2 (4 × 10⁴ spores mL⁻¹) was placed on the leaf surface and after 3 d HR was monitored by staining with lactophenol-trypan blue. B, Plants were infiltrated with suspensions of Psm avrRpm1 (10⁶ cfu mL⁻¹) and after 3 d necrotic lesions were monitored by staining with lactophenol-trypan blue. Bars: 500 μm.