Figure 5.

Expression pattern of PR-1 and GST-1 in the different plant lines. Transcript accumulation was determined by semiquantitative RT-PCR using gene-specific primers and conditions, as described in “Materials and Methods.” A, Determination of PR-1 and GST-1 expression in the different plant lines, without any treatment. Total RNA was extracted from 4-week-old Arabidopsis plants and transcript levels for Actin2 gene, which expression is constitutive, were used to control the amount of mRNA in the reaction. B and C, Time courses of PR-1 and GST-1 expression after Pp Noco2 (B) or Pp Cala2 (C) inoculation. Four-week-old Arabidopsis lines were sprayed with the oomycete spores suspensions and samples for RNA preparations were collected at the indicated times. The values in the histograms denote the relative expression normalized in relation to that of EIF4 gene, in which expression is constitutive.