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. 2007 Mar;143(3):1203–1219. doi: 10.1104/pp.106.092148

Figure 1.

Figure 1.

2-D gels of nonzein proteins extracted from 4-, 14-, and 40-dap maize kernel/endosperm and cuttings of the silver-stained gels at each developmental stage focusing on the seven identified isoforms of PPDK. A, Whole kernels at the seven developmental stages. B, 4-dap kernel 2-DE gel image. C, 14-dap endosperm 2-DE gel image. D, 40-dap endosperm 2-DE gel image. Horizontal streaking due to zeins not focused in the pH range used for IEF is visible on the basic (right) side of the gels. E, Three identified CyPPDK2 isoforms (white crosses, IDs 359, 360, and 357). F, Four identified CyPPDK1 isoforms (white crosses, IDs 399, 398, 388, and 376). The synthetic image composed by Melanie 4 software from the three repeats of the 14-dap stage is shown between one silver-stained gel of the 14-dap developmental stage and the Coomassie Blue-stained gel supporting the 2-D reference map (Méchin et al., 2004). White crosses indicate the corresponding matched spots in each gel cutting.