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. 2007 Mar;143(3):1189–1202. doi: 10.1104/pp.106.094185

Figure 3.

Figure 3.

An alignment of FIP1 primary sequence and a GST member At1g78340 at the amino acid level as well as activity assays of FIP1. A, Alignment of FIP1 with the closest GST member At1g78340 having 67% identity at the amino acid level. B and C, GST activity assays of FIP1 with different concentrations of GSH (B) and CDNB (C) used as substrates. C, Inset indicates 95% purity of the recombinant protein FIP1 used for enzymatic activity assays. M, Protein marker; NI, no IPTG induction; I, with IPTG induction; E1 and E2, eluted protein.