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. 2007 Mar;143(3):1189–1202. doi: 10.1104/pp.106.094185

Figure 4.

Figure 4.

Investigation of phenotype and gene expression of FIP1 transgenic seedlings grown under various light conditions. A, Phenotypes of transgenic seedlings overexpressing or inhibiting FIP1 expression under cFR irradiation. Wild-type Columbia was used as a control. fin219 mutant is another control. FIPOE, FIP1 overexpressor; FIP1AS-171 and FIP1AS-421, FIP1 antisense lines. SALK_080514, T-DNA insertion line of FIP1. Homozygous transgenic seedlings were grown under cFR for 4 d. B, Measurement of hypocotyl length of wild-type Columbia, fin219 mutant (fin219), FIP1 overexpressor (FIP1OE), and FIP1 antisense line (FIP1AS-171 and FIP1AS-421) seedlings grown for 4 d in the dark or under different light conditions. Light intensity, White light (WL) 77 μmol m−2 s−1; FR light, 1.47 μmol m−2 s−1; R light, 93.72 μmol m−2 s−1; and B light, 3.75 μmol m−2 s−1. D, Darkness. Error bars indicate sd (n = 30). C, RNA gel-blot analysis of FIP1 gene expression in transgenic seedlings grown in cFR for 4 d; 20 μg total RNA were loaded onto a gel, then blotted to a nylon membrane (Roche) and probed with a gene-specific region at the 3′-UTR of the FIP1 gene (FIP1 3′-UTR) and the full length of FIP1 cDNA (FIP1 FL).