Figure 12.

Quantification of relative mRNA abundance for genes associated with dormancy or germination in the aleurone layer and embryo of Arabidopsis seeds. Dormant seeds of C24 Arabidopsis were imbibed on water agarose supplemented with or without 200 μm c-PTIO and exposed to water vapors or KCN (300 μm) vapors. RNA abundance in A to H was quantified using qPCR for samples isolated 1, 24, or 48 h after imbibition and is expressed relative to the abundance of reference genes. Asterisks indicate samples where the gene product was not detected.