Figure 5.

The mean growth potential of embryos from dormant Arabidopsis seeds is not changed significantly following treatment of seeds with NO gas or cyanide vapors, or embryos with the NO scavenger c-PTIO. A, Change in relative surface area of the cotyledons or the whole embryo for embryos removed from seeds exposed to air, NO gas, water vapor, or cyanide vapor for 2 d. Isolated embryos were imbibed on agarose containing 300 mOsmol mannitol. Day 1 is 1 d after the end of the treatment period (n > 10). B, Embryos 5 d after isolation from dormant C24 Arabidopsis seeds. Embryos were placed on water agarose without (control) or with 100 μm c-PTIO. [See online article for color version of this figure.]