Figure 9.

Vacuole number per cell is a dynamic parameter in aleurone layers incubated in vitro. The average number of vacuoles per cell between 0 and 4 d after removal of the aleurone layer from an imbibed seed is shown in A for the Col, Kas2, and Cvi ecotypes, and in B for the C24 ecotype of Arabidopsis. The Col ecotype seeds were nondormant, and the Kas2, Cvi, and C24 ecotype seeds were dormant. Aleurone layers in B were incubated on agarose with and without 10 nm ABA. In both A and B, data are presented for that part of the aleurone layer proximal to the root/hypocotyl (root side) and that part proximal to the cotyledons (shoot side).