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. 2007 Mar;143(3):1119–1131. doi: 10.1104/pp.106.093583

Figure 2.

Figure 2.

In vitro prenylation of AGG1 and AGG2. A, Arabidopsis recombinant PFT (lane 2) and PGGT-I (lane 3) were induced and purified from yeast using Anti-Flag antibody (Sigma). Numbers to the left of lane 1 indicate positions of molecular marker (Bio-Rad) in kilodaltons. Positions of protein bands are indicated by the arrows to the α-subunit shared by PFT and PGGT-I, which is tagged with FLAG peptide (PFTA/PGGT-IA, 39 kD), the β-subunit of PFT (PFTB, 54 kD), and the β-subunit of PGGT-I (GGB, 41 kD). B, Purified GST-AGG1, GST-AGG2, GST-AGG1C95S, and GST-AGG2C97S (in which the fourth to last Cys are replaced by Ser), were incubated with (+) or without (−) the purified protein prenyltransferase and tritium-labeled prenyl substrate as indicated in each lane, resolved by SDS-PAGE, and visualized by fluorography.