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. 2007 Mar;143(3):1119–1131. doi: 10.1104/pp.106.093583

Figure 5.

Figure 5.

Immunoblotting using GFP-specific monoclonal antibody. Total (T), soluble (S), and total membrane (M) fraction of protein was prepared from equivalent amounts of Arabidopsis leaf tissue within each sample expressing EYFP-AGG2, AGG2C97S, AGG1, and AGG1C95S as well as EYFP-AGG2 and AGG1 in the background of plp-1, ggb-2, and era1-4 mutants. Intact EYFP-AGG2 in the wild type, as well as in era1-4, was detected in the membrane fraction. EYFP-AGG2C97S was in the cytosol. In the plp-1 mutant, EYFP-AGG2 was detected in both soluble and membrane fractions. In the ggb-2 mutant, the majority of EYFP-AGG2 was detected in the membrane fraction, and a weaker band in the cytosol was shown with longer exposure (as shown in Supplemental Fig. S2). A similar pattern was observed for AGG1.