Figure 4.

Protein synthesis and pre-RC formation in the eIF2αS52A mutant. The cells were synchronized and UV-irradiated as described before measurement of pre-RC formation (A) and protein synthesis (B), both as a function of time (in minutes) after release into the cell cycle. (C) A model of the molecular interactions occurring after UV irradiation to produce a depression of translation and a cell cycle delay. The question mark represents an unidentified factor. The dotted T-shape represents a hypothetical inhibitory activity.